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A. The bar chart corresponds to the mean of three independent proliferation experiments for the 3 TERT + cell lines on one hand and for the 3 ALT + cell lines on the other hand. For each cell line 1200 cells were seeded and proliferation was assessed at days 3, 4 and 5 (Fig. S10). Results presented here show the mean cell count on day 5 ± SD. Mann-Whitney test. ***: p < 0.001. B. Stacked bar chart presenting the result of three independent experiments in which cell fractions in G1, S and G2/M phases of the cell cycle were assessed using <t>propidium</t> iodide labelling for 2 TERT + hybrid cell lines and 3 ALT + cell lines. HBT3 TERT + cell line could not be analyzed because of the presence of two populations with different ploidies. Results correspond to the mean ± SD. Two-way ANOVA with Sidak’s correction for multiple comparisons test. **: p < 0.01 and ****: p < 0.0001. Individual data for each cell line are presented in Fig. S11. C. The bar chart corresponds to the mean of three independent proliferation experiments for the 3 control cell lines (-DOX, in red) and for the 3 cell lines under DOX treatment (+DOX, in blue). Two shRNAs were used (sh2 & sh3). Results are mean ± SD. Unpaired t-test with Welch’s correction. No significant result. BLM inhibition induced by DOX treatment was assessed in parallel by real-time quantitative RT-PCR and western blot (Figs. S12A and S12B). Individual data for each cell line are presented in Fig. S12C.
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A. The bar chart corresponds to the mean of three independent proliferation experiments for the 3 TERT + cell lines on one hand and for the 3 ALT + cell lines on the other hand. For each cell line 1200 cells were seeded and proliferation was assessed at days 3, 4 and 5 (Fig. S10). Results presented here show the mean cell count on day 5 ± SD. Mann-Whitney test. ***: p < 0.001. B. Stacked bar chart presenting the result of three independent experiments in which cell fractions in G1, S and G2/M phases of the cell cycle were assessed using propidium iodide labelling for 2 TERT + hybrid cell lines and 3 ALT + cell lines. HBT3 TERT + cell line could not be analyzed because of the presence of two populations with different ploidies. Results correspond to the mean ± SD. Two-way ANOVA with Sidak’s correction for multiple comparisons test. **: p < 0.01 and ****: p < 0.0001. Individual data for each cell line are presented in Fig. S11. C. The bar chart corresponds to the mean of three independent proliferation experiments for the 3 control cell lines (-DOX, in red) and for the 3 cell lines under DOX treatment (+DOX, in blue). Two shRNAs were used (sh2 & sh3). Results are mean ± SD. Unpaired t-test with Welch’s correction. No significant result. BLM inhibition induced by DOX treatment was assessed in parallel by real-time quantitative RT-PCR and western blot (Figs. S12A and S12B). Individual data for each cell line are presented in Fig. S12C.

Journal: bioRxiv

Article Title: Alternative Lengthening of Telomeres and CINSARC are interconnected toward non-translocation-related sarcomas progression

doi: 10.64898/2026.01.23.701253

Figure Lengend Snippet: A. The bar chart corresponds to the mean of three independent proliferation experiments for the 3 TERT + cell lines on one hand and for the 3 ALT + cell lines on the other hand. For each cell line 1200 cells were seeded and proliferation was assessed at days 3, 4 and 5 (Fig. S10). Results presented here show the mean cell count on day 5 ± SD. Mann-Whitney test. ***: p < 0.001. B. Stacked bar chart presenting the result of three independent experiments in which cell fractions in G1, S and G2/M phases of the cell cycle were assessed using propidium iodide labelling for 2 TERT + hybrid cell lines and 3 ALT + cell lines. HBT3 TERT + cell line could not be analyzed because of the presence of two populations with different ploidies. Results correspond to the mean ± SD. Two-way ANOVA with Sidak’s correction for multiple comparisons test. **: p < 0.01 and ****: p < 0.0001. Individual data for each cell line are presented in Fig. S11. C. The bar chart corresponds to the mean of three independent proliferation experiments for the 3 control cell lines (-DOX, in red) and for the 3 cell lines under DOX treatment (+DOX, in blue). Two shRNAs were used (sh2 & sh3). Results are mean ± SD. Unpaired t-test with Welch’s correction. No significant result. BLM inhibition induced by DOX treatment was assessed in parallel by real-time quantitative RT-PCR and western blot (Figs. S12A and S12B). Individual data for each cell line are presented in Fig. S12C.

Article Snippet: DNA content was quantified with propidium iodide fluorescence intensity by flow cytometry (MACSQuant VYB, Miltenyi Biotec) and analyzed using the cycle tool of the FlowJo software (RRID:SCR_008520, v10.10.0, FlowJo LLC) and GraphPad Prism (RRID:SCR_002798, v6.01, GraphPad Software Inc.) software.

Techniques: Cell Characterization, MANN-WHITNEY, Control, Inhibition, Quantitative RT-PCR, Western Blot